One Step Nucleic Acid Amplification (OSNA) Lysate Samples Are Suitable to Establish a Transcriptional Metastatic Signature in Patients with Early Stage Hormone Receptors-Positive Breast Cancer
The One Step Nucleic Acid Amplification (OSNA) is a recent technique for sentinel lymph nodes staging in breast cancer (BC). After OSNA assay, instead of being discarded, the residual OSNA lysate can also be used for gene expression studies, being the potentially ideal sample to search for new biomarkers. The aim of our study was to identify biomarkers related to tumor-microenvironment interplay in OSNA lysate of sentinel lymph nodes in women with early stage hormone receptors-positive BC. We identified 11 upregulated genes in metastatic lymph nodes. These genes codify proteins mainly involved in cancer aggressiveness and with impact in immune response. Thus, these findings support that OSNA lysate transcriptomic analysis may identify biomarkers potentially useful in the future for prognosis stratification and therapy selection. As OSNA assay is being implemented for sentinel lymph nodes staging in other cancers, this approach could also have a wider utility.
Authors and Affiliations:
Inês Gante 1,2,3,Joana Martins Ribeiro 4, João Mendes 3,4, Ana Gomes 5, Vânia Almeida 5,6, Frederico Soares Regateiro 3,7,8, Francisco Caramelo 3,9,10, Henriqueta Coimbra Silva 3,4,10 and Margarida Figueiredo-Dias 1,2,3
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